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KMID : 1140120110160040294
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Cancer Prevention Research
2011 Volume.16 No. 4 p.294 ~ p.301
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Inter-Laboratory Validation of in vivo Comet Assay in Korea
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Kwon Jee-Young
Kim Ji-Young
Chung Moon-Koo
Kim Yun-Soon
Che Jeong-Hwan
Oh Sang-Suk
Hong Eun-Kyung
Oh Sang-Woo
Kim Joo-Hwan
Yum Young-Na
Sohn Soo-Jung
Seo Young-Rok
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Abstract
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The comet assay can be used to investigate the genotoxicity of various agents including industrial chemicals, biocides, agrochemicals and pharmaceuticals. Nowadays, international expert groups for comet assay have published recommendations describing standards which are aimed at establishing high quality protocols in order to obtain valid and reliable data. Despite that the international activity for validation of in vivo comet assay has been carried out, the distribution of protocol and interlaboratory validation has not yet conducted in Republic of Korea. Here, three laboratories participated in interlaboratory assessment for the test validity of the in vivo comet assay under the GLP (Good Laboratory Practice) system. Two genotoxic chemicals, N-methyl-N-nitrosourea (MNU) and ethyl methansulfonate (EMS),were applied as known and unknown chemicals, respectively, which were selected on the basis of the 3rd international validation study result. Among all GLP laboratories, the results showed a significant increase in DNA damage as % of tail DNA as dose-related manner in both liver and stomach tissues treated with MNU relative to negative control group. Similarly, EMS-treated group also showed significant incremental % of tail DNA as dose-dependent manner compared with negative control group in both tissues. The in vivo comet results from all GLP laboratories in Republic of Korea were obviously consistent with the 3rd international validation study. In conclusion, we confirmed the interlaboratory validity of in vivo comet assay using two genotoxic chemicals, which was assessed by three GLP laboratories in Republic of Korea. This study can strengthen in vivo comet assay to be suggested as an international standard protocol or guideline for genotoxicity test of various genotoxic agents.
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KEYWORD
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In vivo comet assay, Interlaboratory validation, Good laboratory practice (GLP) system
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